Adapting spa typing for national laboratory-based surveillance of methicillin-resistant Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Classifying spa types in complexes improves interpretation of typing results for methicillin-resistant Staphylococcus aureus.
A total of 382 isolates of methicillin-resistant Staphylococcus aureus originating from three Austrian regions and one adjacent Italian region (Vienna, Lower Austria, North Tyrol, and South Tyrol) were typed by DNA sequence analysis of the variable repeat region of the protein A gene (spa typing). The strain collection consisted of arbitrarily chosen isolates originating from clinical specimens...
متن کاملEvaluation of spa-typing of methicillin-resistant Staphylococcus aureus using high-resolution melting analysis.
OBJECTIVE spa-typing of methicillin-resistant Staphylococcus aureus (MRSA) has been used widely in clinical diagnostics and epidemiological studies. The aim of this study was to evaluate high-resolution melting (HRM) as a rapid and cost-effective method, to replace DNA-sequencing, for spa-typing in a global collection of 50 MRSA isolates. METHODS The polymorphic X region of the spa gene was a...
متن کاملMolecular typing of methicillin-resistant Staphylococcus aureus strains by PCR-RFLP of SPA gene: a reference laboratory perspective.
PURPOSE To characterize methicillin-resistant Staphylococcus aureus (MRSA) strains by molecular typing based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of spa gene and to assess the utility of spa genotyping over bacteriophage typing in the discrimination of the strains. MATERIALS AND METHODS Studies were undertaken on 125 MRSA strains representing the mo...
متن کامل[Multilocus sequence typing of methicillin-resistant Staphylococcus aureus].
OBJECTIVE To analyze multilocus sequence typing (MLST) of methicillin-resistant Staphylococcus aureus (MRSA) strains in 2000 and 2005, and get a primary knowledge of MLST Characterization of MRSA. METHODS Sequence analysis was conducted on seven allelic genes of 29 methicillin-resistant Staphylococcus aureus strains and 2 methicillin-sensitive Staphylococcus aureus (MSSA) strains and the alle...
متن کاملDetection anD typing methoDs of methicillin-resistant StaphylococcuS aureuS strains
Staphylococcus aureus is one of the most important pathogens in hospital infections and its importance in the community is also prominent, especially since the 1990’s. Today methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of hospital infection, causing 40-70% of staphylococcal infections in intensive care units. Data on the prevalence of MRSA isolates differ ...
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ژورنال
عنوان ژورنال: European Journal of Clinical Microbiology & Infectious Diseases
سال: 2011
ISSN: 0934-9723,1435-4373
DOI: 10.1007/s10096-011-1158-5